Tag: differential expression analysis
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RNA-Seq Analysis in WSL – Part 3 : Differential expression analysis using Ballgown
Once we create the transcript abundance data using Stringtie, we can now perform the differential expression analysis. With Strigtie, we had created the estimated transcript abundance data which is compatible with Ballgown. The directory structure of the `for_ballgown` directory looks like this: To perform the differential gene expression analyis open Rstudio and change working directory…